Livro O Segredo Das Loterias Pdf 25 [BEST]

Livro O Segredo Das Loterias Pdf 25 [BEST]

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Livro O Segredo Das Loterias Pdf 25

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Bioengineering 2012

August 16, 2012. My name is Charles Edward. I’m a first year biology student at the University of Oregon and my research, carried out during a summer internship with Joshua Winicki, has been published in the journal General and Comparative Endocrinology.

In a study published earlier this year, Joshua Winicki and I showed that the grasshopper Chorthippus japonicus expresses two sets of insulin-like growth factors, one somatogenic and the other autogenic. The researchers also found that they could manipulate the growth and reproduction of the animals through application of the somatogenic hormone. In the following studies, we sought to identify the regulatory signals that indicate when and where the somatogenic signal is active.

For these studies, we used electroporation, in which DNA is delivered to individual cells through brief electroporation. DNA was delivered to the somatogenic cells of grasshopper embryos using an existing transgenic line which carries the green fluorescent protein as a marker. We observed that the markers visible in somatogenic cells in the grasshopper embryo were labile. Thus, we designed experiments to measure the half-life of the protein markers, in order to attempt to quantify the rate at which DNA from the somatogenic cells is lost, and to track the movement of such DNA into and out of somatogenic cells.

We were able to establish a half-life of 16 minutes for the red fluorescent protein marker in vivo, but could not measure the half-life of the green fluorescent protein because it could only be used for morphological studies. In vitro experiments, using the marker in the form of plasmid DNA rather than RNA, suggested that the protein was degraded at a rate of 22 minutes. We predicted that if the somatogenic cells were nonautoregulatory and had no need for their own growth factor, the marker would be continuously produced and degraded, and would be present at a constant low level. Our electroporation studies showed that the DNA was degraded at a slightly lower rate than predicted, suggesting that there is some posttranscriptional control on the degradation of these proteins.

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